Real-time investigation of dynamic protein crystallization in living cells

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Molecularesgl
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Moleculargl
dc.contributor.authorSchonherr, R.
dc.contributor.authorKlinge, M.
dc.contributor.authorRudolph, J. M.
dc.contributor.authorFita, K.
dc.contributor.authorRehders, D.
dc.contributor.authorLubber, F.
dc.contributor.authorSchneegans, S.
dc.contributor.authorMajoul, I. V.
dc.contributor.authorDuszenko, M.
dc.contributor.authorBetzel, C.
dc.contributor.authorBrandariz Núñez, Alberto
dc.contributor.authorMartínez Costas, José Manuel
dc.contributor.authorDuden, R.
dc.contributor.authorRedecke, L.
dc.date.accessioned2020-05-05T11:49:54Z
dc.date.available2020-05-05T11:49:54Z
dc.date.issued2015
dc.description.abstractX-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.gl
dc.description.peerreviewedSIgl
dc.description.sponsorshipR.S. thanks the Graduate School for Computing in Medicine and Life Sciences at the University of Lübeck for funding. L.R., M.K., D.R., and C.B. thank the German Federal Ministry for Education and Research (BMBF) for funding (Grant Nos. 01KX0806 and 01KX0807). L.R., M.D., and C.B. acknowledge support from the BMBF in the context of the Röntgen-Angström-Cluster (Grant No. 05K12GU3). J.M.-C. and A.B.-N. acknowledge support from the Spanish Ministerio Economía y Competitividad (MINECO, Grant No. BFU2013-43513-R). I.V.M., R.D., and L.R. are grateful for support from the DFG Cluster of Excellence “Inflammation at Interfaces” (EXC 306)gl
dc.identifier.citationSchonherr, R., Klinge, M., Rudolph, J.M. et al. (2015). Real-time investigation of dynamic protein crystallization in living cells, "Structural Dynamics" 2, 041712gl
dc.identifier.doi10.1063/1.4921591
dc.identifier.issn2329-7778
dc.identifier.urihttp://hdl.handle.net/10347/22034
dc.language.isoenggl
dc.publisherAIP Publishinggl
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2013-43513-R/ES/REOVIRUS AVIAR: FACTORES DE VIRULENCIA Y NUEVAS DIANAS TERAPEUTICAS
dc.relation.publisherversionhttps://doi.org/10.1063/1.4921591gl
dc.rightsCopyright © 2015, Author(s). This article is distributed under a Creative Commons Attribution 3.0 Unported licensegl
dc.rights.accessRightsopen accessgl
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.titleReal-time investigation of dynamic protein crystallization in living cellsgl
dc.typejournal articlegl
dc.type.hasVersionVoRgl
dspace.entity.typePublication
relation.isAuthorOfPublicationf55dab55-97e6-4c7d-9b63-71097cbf7aa4
relation.isAuthorOfPublication.latestForDiscoveryf55dab55-97e6-4c7d-9b63-71097cbf7aa4

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